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ASOlutions™

Precision ASO Design for RNA Therapeutics

Computational ASO design services powered by ASOwalker™ — developed by RNA scientists with direct experience in FDA-approved ASO drug programs.

5
ASO Mechanisms
100%
Virtual / Global
PhD
RNA Expertise

The Challenge

ASO design traditionally relies on approaches that are slow, expensive, and fragmented.

Trial and error

Synthesizing dozens of candidates hoping a few work

Scattered expertise

Deep knowledge locked in pharma silos

Slow iteration

Months of optimization per program

What We Deliver

Target Analysis

Complete gene/transcript characterization, isoform mapping, exon-intron architecture

ASO Candidates

Ranked, optimized sequences with clear scientific rationale for each recommendation

Off-Target Report

Transcriptome-wide specificity analysis with expression-weighted risk stratification

Chemistry Recommendations

Backbone, sugar modifications, and architecture optimized for your mechanism and target tissue

Synthesis-Ready Files

Sequences formatted for direct vendor ordering (IDT, Dharmacon, Eurogentec, etc.)

Five ASO Mechanisms Supported

1

RNase H Knockdown

Gapmer designs for mRNA degradation

2

Exon Skipping

Block splice enhancers to skip disease-causing exons

3

Exon Inclusion

Block splice silencers to restore exon inclusion

4

Translation Blocking

Steric block of ribosome binding

5

Expression Enhancement

Target upstream regulatory elements to increase protein

Service Tiers

Flexible options for every stage of your ASO program.

Basic Screening

$500 – $1,000

Target feasibility assessment

Single target gene analysis
ASO candidate generation (3-5 sequences)
Basic off-target screening
Design rationale summary
1 revision round

Best for: Academic labs, early feasibility studies

Most Popular

Standard Design

$2,000 – $3,000

Comprehensive ASO strategy

Full target gene analysis with isoform mapping
Multiple ASO candidates (5-10 sequences)
Comprehensive off-target analysis
Thermodynamic predictions
Chemistry optimization recommendations
Detailed design report
3 revision rounds

Best for: Biotech R&D, serious lead identification

Advanced Consulting

$4,000+

Enterprise partnership

Multi-target panels
Full candidate library (10-20+ sequences)
Advanced splicing analysis
Delivery strategy consultation
Chemistry optimization deep-dive
Synthesis partner coordination
Priority support
Unlimited revisions

Best for: Pharma programs, IND-enabling work

Our Process

1

Consultation

Discuss your target, goals, and timeline

Free
2

Target Analysis

Comprehensive gene/transcript characterization

1-2 weeks
3

ASO Design

Generate and rank optimized candidates

1-2 weeks
4

Validation Report

Off-target analysis, chemistry recommendations

1 week
5

Delivery

Synthesis-ready files, detailed documentation

Final
6

Support

Revision rounds, ongoing consultation

Included

What Makes Us Different

Deep RNA Expertise

Founded by a PhD molecular biologist trained in Alberto Kornblihtt's lab (UBA/CONICET) — a global leader in RNA splicing. Direct collaboration with Dr. Adrian Krainer's group, pioneers of Nusinersen.

Computational Precision

Our ASOwalker™ platform integrates multiple layers of biological evidence and validated design principles to systematically evaluate and rank ASO candidates with high confidence.

Mechanism Expertise

We don't just generate sequences — we understand why they should work. Every recommendation comes with scientific rationale grounded in published literature.

Global Service

100% virtual consultancy. We serve clients worldwide with seamless collaboration across time zones.

Patient-Driven Mission

Rooted in work with patient advocacy groups (FAME Argentina, CureSMA). We understand the urgency of rare disease drug development.

Who We Serve

Academic Research Labs

Gene function studies
Disease model development
RNA biology research
Cost-effective solutions

Biotech & Pharma

Early-stage drug discovery
Target validation
Lead optimization
Pipeline acceleration

Rare Disease Foundations

Patient advocacy partnerships
Orphan drug development
Personalized ASO design
Compassionate access support

Ready to Accelerate Your ASO Program?

Free consultation — discuss your project, no obligation. We'll help you understand what's possible and what it takes.

Web: www.asolutions-therapeutics.com

© 2026 ASOlutions™. ASOlutions and ASOwalker are trademarks. This document is for informational purposes and does not constitute medical advice.